Journal: Current Protocols

Article Title: A Guide to Enzymatic Targeted Methylation Sequencing Bioinformatics Workflow: Best Practices

doi: 10.1002/cpz1.70464

Figure Lengend Snippet: Input and Output of each stage

Article Snippet: Targeted methylation profiling was performed using the NEBNext EM‐seq Kit combined with the Twist Targeted Methylation Sequencing platform (Twist Bioscience, USA), following the manufacturer's instructions (New England Biolabs, ; “Twist Targeted Methylation Sequencing Protocol | Twist Bioscience,” accessed July 30, 2025).

Techniques: Sequencing, Methylation, Analysis, Control, Tiling

Journal: Current Protocols

Article Title: A Guide to Enzymatic Targeted Methylation Sequencing Bioinformatics Workflow: Best Practices

doi: 10.1002/cpz1.70464

Figure Lengend Snippet: Estimated duration of the complete analysis

Article Snippet: Targeted methylation profiling was performed using the NEBNext EM‐seq Kit combined with the Twist Targeted Methylation Sequencing platform (Twist Bioscience, USA), following the manufacturer's instructions (New England Biolabs, ; “Twist Targeted Methylation Sequencing Protocol | Twist Bioscience,” accessed July 30, 2025).

Techniques: Analysis, QC/EQA/Proficiency, Sequencing, Methylation, Tiling

Journal: iScience

Article Title: Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke

doi: 10.1016/j.isci.2026.116257

Figure Lengend Snippet: Cherp serving an effective treatment target for IS (A) The neuron counts of cluster 3 and 5. (B) The Cherp expression in each cluster. (C) The WB image of Cherp expression after OGD/R process. (D) The IF images of Cherp expression after OGD/R process. Scale bars = 100 μm. (E) The efficiency validation of Cherp knocking down using shRNA. (F) The cell viability assay after Cherp knocking down. (G) LDH leakage assay after Cherp knocking down. (H) TUNEL assay after Cherp knocking down. Scale bars = 100 μm. (I) WB image of apoptosis-related markers after Cherp knocking down. (J) Morphology of cerebral edema in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery. (K) TTC staining of cerebral infarction in sham, MCAO and MCAO-shRNA intervention groups at 72 h post-surgery (Dashed area represents the infarcted brain region). (L) Statistics analysis of brain water content ( n = 5 mice/group). (M) Quantification of infarct area ( n = 5 mice/group). (N) The functional enrichment of Cherp high cells related pathways. (O) The intracellular calcium imaging after Cherp knocking down. Scale bars = 100 μm (P) Quantitative analysis of intracellular Ca 2+ fluorescence intensity in primary neurons under indicated conditions ( n = 3 independent experiments). Data are presented as mean ± SD. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 and ∗∗∗∗ p < 0.0001 as determined by Student’s t test.

Article Snippet: The shRNA sequences targeting Cherp were generated at Sangon Biotech.

Techniques: Expressing, Biomarker Discovery, shRNA, Viability Assay, TUNEL Assay, Staining, Functional Assay, Imaging, Fluorescence